Restriction Enzymes
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Filtered Search Results
Thermo Scientific™ FastDigest ApaI
Cut at GGGCC^C sites with Thermo Scientific™ FastDigest™ ApaI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PspOMI).
Thermo Scientific™ FastDigest PvuI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ SgrDI (5 U/μL)
Cut at CG^TCGACG sites with SgrDI restriction enzyme, which functions best at 37°C in R buffer.
Thermo Scientific™ GTP Solution (100 mM)
Thermo Scientific GTP (guanosine 5'-triphosphate) is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Thermo Scientific™ FastDigest MunI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
| Sensitive to Heat Inactivation | No |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | MunI |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest RsaI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest BspTI
Cut at C^TTAAG sites with Thermo Scientific™ FastDigest™ AflII (BspTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest Eco32I
Cut at GAT^ATC sites with Thermo Scientific™ FastDigest™ EcoRV (Eco32I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: EcoRV).
| Sensitive to Heat Inactivation | No |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Eco32I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | Blunt |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest CsiI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ HindIII (10 U/μL)
The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ PfoI (10 U/μL)
Cut at T^CCNGGA sites with PfoI restriction enzyme recognizes and cuts best at 37°C in Tango buffer.
Thermo Scientific™ SmaI (10 U/μL)
Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ LguI (SapI) (5 U/μL)
The LguI (SapI) restriction enzyme recognizes GCTCTTC(1/4)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: BspQI, PciSI, SapI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | LguI (SapI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Scarless Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | Yes |
| Optimal Reaction Temperature | 37°C |
| Concentration | 5 U/μL |
Thermo Scientific™ FastDigest BstXI
Cut at CCANNNNN^NTGG sites with Thermo Scientific™ FastDigest™ BstXI, which performs best at 55°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest Mph1103I
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.